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sigG [2018-04-30 08:46:29]
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sigG [2018-04-30 08:46:29]

RNA polymerase sporulation forespore-specific (late) sigma factor SigG
locus
BSU15330
pI
5.50
mw
29.92 kDa
protein length
260 aa Sequence Blast
gene length
780 bp Sequence Blast
function
transcription of sporulation genes (late forespore)
product
RNA polymerase sporulation forespore-specific (late) sigma factor SigG
essential
no
synonyms
spoIIIG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    1,605,630 → 1,606,412

    The protein

    Protein family

  • sigma-70 factor family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]

    • Effectors of protein activity

  • [protein|01412FA454DDBA12622E3B32C433F8059123661B|CsfB] inhibits SigG activity [Pubmed|19497328]
  • the activity of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]-containing [SW|RNA polymerase] is modulated by [protein|8AB50581EBB3C27BB9A96EA3382F8C90CE5B6EC5|YlyA] [Pubmed|23678950]

    • Expression and Regulation

    Operons

    genes
    [gene|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|spoIIGA]-[gene|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]-[gene|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]
    description
    [Pubmed|1902213]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2512576], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|1902213], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

    • regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|8288522,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • ''[SW|spoIIGA]'': expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8288522,15687200 PubMed]

    • additional information

  • the mRNA half-life is about 2.6 min [PubMed|24163345]
    • view in new tab

    genes
    [gene|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]
    description
    [Pubmed|1902213]

    regulation

  • ''[SW|spoIIGA]'': expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8288522,15687200 PubMed]

    • additional information

  • the mRNA half-life is about 2.6 min [PubMed|24163345]
  • a conserved hairpin in the 5' leader sequence of the [gene|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] mRNA occludes the ribosome-binding site, this reduces translation by up to 4-fold [pubmed|29702640]
    • view in new tab

    Biological materials

    Mutant

  • BKE15330 (Δ[gene|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTTCCCTCCCTACA, downstream forward: _UP4_TAATGAAAAGCCTTTAAAAC
  • BKK15330 (Δ[gene|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTTCCCTCCCTACA, downstream forward: _UP4_TAATGAAAAGCCTTTAAAAC

    • Labs working on this gene/protein

  • [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

    • References

    Reviews

  • 20833318

    • The [SW|SigG regulon]

  • 16497325

    • Original Publications

  • 1900507,1902463,15175314,10806362,1902213,2497051,12867473,16707705,9829949,1487728,2448286,9852018,19497328,8126438,8355618,24163345,18820020,2493633,7961403,15256570,12813085,9004507,8885263,18485064,19390092,23678950,22984259,20817675,21935351,25835496